NAD+

(Nicotinamide Adenine Dinucleotide) – Research Coenzyme for Cellular Metabolism and Energy Studies

Introduction to NAD+

NAD+ (Nicotinamide Adenine Dinucleotide) is a critical cellular coenzyme present in all living cells. It plays a central role in energy metabolism, redox reactions, and enzymatic signaling, making it a key molecule in laboratory and preclinical research.

Researchers use this compound to study mitochondrial function, oxidative stress, and age-related cellular processes, providing insight into metabolic regulation and cellular resilience.

What Is NAD+?

This molecule is a dinucleotide composed of adenine and nicotinamide, connected through phosphate groups. In its oxidized form (NAD+), it accepts electrons during metabolic reactions, and its reduced form (NADH) donates electrons to support ATP production and maintain redox balance.

Due to its essential role in energy metabolism, this coenzyme is often applied in cell culture studies, enzymatic assays, and mitochondrial biology research.

Role in Cellular Metabolism

NAD+ serves as a key regulator of metabolic pathways, including glycolysis, the citric acid cycle, and oxidative phosphorylation. It also functions as a substrate for enzymes such as sirtuins and poly(ADP-ribose) polymerases (PARPs), which are involved in DNA repair, gene regulation, and stress-response pathways.

Laboratory research demonstrates its involvement in:

• Energy production and mitochondrial function
• Redox balance and oxidative stress mitigation
• Regulation of sirtuin-dependent signaling
• DNA repair and genomic stability
• Age-related cellular adaptation

These properties make it an indispensable tool in metabolic, aging, and molecular biology studies.

Research Applications

The compound is commonly used to:

• Study metabolic and mitochondrial pathways
• Investigate cellular responses to oxidative stress
• Explore age-associated molecular changes
• Evaluate enzymatic activity in vitro
• Model disease mechanisms related to energy and redox balance

Using high-purity material ensures consistent, reproducible results in experimental studies.

Product Specifications

• Name: NAD+ (Nicotinamide Adenine Dinucleotide)
• Category: Coenzyme / Research compound
• Purity: ≥99% (HPLC-verified)
• Form: Lyophilized powder or crystalline solid
• Appearance: White to off-white solid
• Solubility: Soluble in water, PBS, or compatible laboratory buffers

Handling and Storage

• Store at −20°C or below, protected from light
• Reconstitute under sterile laboratory conditions
• Prepare aliquots to avoid repeated freeze–thaw cycles
• Minimize exposure to oxygen and moisture

Following these practices maintains biological activity and experimental consistency.

Research-Only Use

This molecule is intended strictly for laboratory research. It is not approved for human or veterinary use, nor for therapeutic, diagnostic, or cosmetic purposes. Experiments should only be conducted by trained professionals in appropriate laboratory settings.

Why This Coenzyme Is Valuable in Research

NAD+ provides insights into cellular energy metabolism, redox signaling, and mitochondrial function, making it critical for studies on aging, metabolic regulation, and disease modeling. Researchers rely on its well-characterized properties and reproducibility for reliable experimental outcomes.